Fig. 4. High levels of both miR-675-5' and 3' are expressed during myoblast differentiation. TaqMan RT-qPCR for miR-675-5' and 3' (A) and SYBR RT-qPCR for H19 (B) and MMP1 (C) in two samples of aSkM tissue and three series of differentiation of skeletal myotubes. aSkM, adult skeletal muscle; SkMyot, skeletal myotubes. The two data points for aSkM originate from two RNA preparations from two different commercial suppliers.